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酿酒酵母异质性菌株中线粒体基因组的结构与转录

Structure and transcription of the mitochondrial genome in heteroplasmic strains of Saccharomyces cerevisiae.

作者信息

Kang Y W, Miller D L

机构信息

Biology Program, University of Texas, Richardson 75083-0688.

出版信息

Nucleic Acids Res. 1989 Nov 11;17(21):8595-609. doi: 10.1093/nar/17.21.8595.

DOI:10.1093/nar/17.21.8595
PMID:2479907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC335030/
Abstract

Saccharomyces cerevisiae strain FF1210-6C/170 is respiratory deficient due to a mutation of the penultimate base of the mitochondrial tRNA(Asp) gene. We have identified a number of progeny from this strain which have reverted to respiratory sufficiency by the excision and tandem amplification of a small region of the mitochondrial (mt) DNA carrying the tRNA(Asp) gene, while also maintaining the full-length mtDNA. We have studied the structure of the mtDNA and mitochondrial transcription in a number of these heteroplasmic strains. The exact site of the recombination involved in the excision of the repeating unit of the amplified mtDNA has been determined for five of the revertants. Recombination occurs between identical sequences 4-13 base pairs in length. Each of the different repeating units of the amplified DNA retains an active promoter which has been moved to a site just upstream of the tRNA(Asp) gene by the excision/amplification. Transcripts from the heteroplasmic strains have been characterized to determine the sites of mitochondrial RNA termini. We find that in addition to the 5' and 3' processing of the tRNAs, many of the transcripts terminate at a position about 300 base pairs downstream of the gene for tRNA(Asp). We also find that 3' processing of tRNA(Asp) precursors is absent in petite strains which lack 5' processing indicating that 5' processing of tRNA(Asp) may be a prerequisite for 3' processing in this mutant.

摘要

酿酒酵母菌株FF1210 - 6C/170因线粒体天冬氨酸转运RNA（tRNAAsp）基因倒数第二个碱基发生突变而呼吸缺陷。我们已鉴定出该菌株的一些子代，它们通过切除并串联扩增携带tRNAAsp基因的线粒体（mt）DNA小区域而恢复为呼吸充足，同时还保留了全长mtDNA。我们研究了许多这些异质菌株中的mtDNA结构和线粒体转录。已确定五个回复突变体中涉及扩增mtDNA重复单元切除的重组确切位点。重组发生在长度为4 - 13个碱基对的相同序列之间。扩增DNA的每个不同重复单元都保留一个活性启动子，该启动子通过切除/扩增已移动到tRNAAsp基因上游的一个位点。已对异质菌株的转录本进行了表征，以确定线粒体RNA末端的位点。我们发现，除了tRNA的5'和3'加工外，许多转录本在tRNAAsp基因下游约300个碱基对的位置终止。我们还发现，在缺乏5'加工的小菌落菌株中不存在tRNAAsp前体的3'加工，这表明tRNAAsp的5'加工可能是该突变体中3'加工的先决条件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0588/335030/4bdeb6cfd263/nar00138-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0588/335030/5956858526e5/nar00138-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0588/335030/a8972df73f22/nar00138-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0588/335030/4bdeb6cfd263/nar00138-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0588/335030/5956858526e5/nar00138-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0588/335030/a8972df73f22/nar00138-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0588/335030/4bdeb6cfd263/nar00138-0205-a.jpg

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引用本文的文献

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A missense mutation in the nuclear gene coding for the mitochondrial aspartyl-tRNA synthetase suppresses a mitochondrial tRNA(Asp) mutation.编码线粒体天冬氨酰 - tRNA合成酶的核基因中的错义突变抑制了线粒体tRNA（Asp）突变。
Nucleic Acids Res. 2000 Apr 1;28(7):1542-7. doi: 10.1093/nar/28.7.1542.
2
Ordered processing of the polygenic transcripts from a mitochondrial tRNA gene cluster in K. lactis.乳酸克鲁维酵母中线粒体tRNA基因簇多基因转录本的有序加工。
Curr Genet. 1994 Apr;25(4):342-9. doi: 10.1007/BF00351488.
3
Novel hybrid maturases in unstable pseudorevertants of maturaseless mutants of yeast mitochondrial DNA.

本文引用的文献

1
Novel mechanisms for maturation of chloroplast transfer RNA precursors.叶绿体转移 RNA 前体成熟的新机制。
EMBO J. 1988 Jun;7(6):1567-74. doi: 10.1002/j.1460-2075.1988.tb02981.x.
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Transcripts of mitochondrial tRNA genes in Saccharomyces cerevisiae.酿酒酵母中线粒体tRNA基因的转录本
酵母线粒体DNA无成熟酶突变体不稳定假回复体中的新型杂合成熟酶
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Location and structure of the var1 gene on yeast mitochondrial DNA: nucleotide sequence of the 40.0 allele.酵母线粒体DNA上var1基因的定位与结构：40.0等位基因的核苷酸序列
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5
Organization and processing of the mitochondrial oxi3/oli2 multigenic transcript in yeast.酵母中线粒体oxi3/oli2多基因转录本的组织与加工
Mol Gen Genet. 1984;196(2):266-74. doi: 10.1007/BF00328059.
6
Assembly of the mitochondrial membrane system. CBP1, a yeast nuclear gene involved in 5' end processing of cytochrome b pre-mRNA.线粒体膜系统的组装。CBP1，一个参与细胞色素b前体mRNA 5'端加工的酵母核基因。
J Biol Chem. 1984 Apr 25;259(8):4722-31.
7
Characterization of a yeast mitochondrial locus necessary for tRNA biosynthesis. Deletion mapping and restriction mapping studies.tRNA生物合成所需的酵母线粒体基因座的特征分析。缺失图谱绘制和限制性图谱绘制研究。
Mol Gen Genet. 1983;191(3):512-8. doi: 10.1007/BF00425771.
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Excision sequences in the mitochondrial genome of yeast.酵母线粒体基因组中的切除序列。
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A nuclear mutation that post-transcriptionally blocks accumulation of a yeast mitochondrial gene product can be suppressed by a mitochondrial gene rearrangement.一种转录后阻断酵母线粒体基因产物积累的核突变可被线粒体基因重排所抑制。
J Mol Biol. 1984 Jun 5;175(4):431-52. doi: 10.1016/0022-2836(84)90178-5.
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Processing of yeast mitochondrial messenger RNAs at a conserved dodecamer sequence.酵母线粒体信使核糖核酸在保守的十二聚体序列处的加工
EMBO J. 1984 Apr;3(4):829-34. doi: 10.1002/j.1460-2075.1984.tb01892.x.