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在紫外线诱变中存在缺陷的uvsI突变体确定了曲霉属中uvs基因的第四个上位性基因群。

uvsI mutants defective in UV mutagenesis define a fourth epistatic group of uvs genes in Aspergillus.

作者信息

Chae S K, Kafer E

机构信息

Department of Biology, McGill University, Montreal, Canada.

出版信息

Curr Genet. 1993 Jul-Aug;24(1-2):67-74. doi: 10.1007/BF00324667.

Abstract

Three UV-sensitive mutations of A. nidulans, uvsI, uvsJ and uvsA, were tested for epistatic relationships with members of the previously established groups, here called the "UvsF", "UvsC", and "UvsB" groups. uvsI mutants are defective for spontaneous and induced reversion of certain point mutations and differ also for other properties from previously analyzed uvs types. They are very sensitive to the killing effects of UV-light and 4-NQO (4-nitro-quinoline-N-oxide) but not to MMS (methylmethane sulfonate). When double- and single-mutant uvs strains were compared for sensitivity to these three agents, synergistic or additive effects were found for uvsI with all members of the three groups. The uvsI gene may therefore represent a fourth epistatic group, possibly involved in mutagenic repair. On the other hand, uvsJ was clearly epistatic with members of the UvsF group and fitted well into this group also by phenotype. The uvsA gene was tentatively assigned to the UvsC group. uvsA showed epistatic interactions with uvsC in all tests, and like UvsC-group mutants is UV-sensitive mainly in dividing cells. However, the uvsA mutation does not cause the defects in recombination and UV mutagenesis typical for this group.

摘要

对构巢曲霉的三个紫外线敏感突变体uvsI、uvsJ和uvsA,测试了它们与先前确定的组(这里称为“UvsF”、“UvsC”和“UvsB”组)成员之间的上位关系。uvsI突变体在某些点突变的自发和诱导回复方面存在缺陷,并且在其他特性上也与先前分析的uvs类型不同。它们对紫外线和4-硝基喹啉-N-氧化物(4-NQO)的杀伤作用非常敏感,但对甲基磺酸甲酯(MMS)不敏感。当比较双突变和单突变uvs菌株对这三种试剂的敏感性时,发现uvsI与三组的所有成员都有协同或加性效应。因此,uvsI基因可能代表第四个上位组,可能参与诱变修复。另一方面,uvsJ与UvsF组的成员明显呈上位关系,并且在表型上也很好地符合该组。uvsA基因暂时归入UvsC组。在所有测试中,uvsA与uvsC都表现出上位相互作用,并且与UvsC组突变体一样,主要在分裂细胞中对紫外线敏感。然而,uvsA突变不会导致该组典型的重组和紫外线诱变缺陷。

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