Han K Y, Chae S K, Han D M
Dept. of Molecular Biology, Wonkwang University, Iksan, South Korea.
FEMS Microbiol Lett. 1998 Jul 1;164(1):13-9. doi: 10.1111/j.1574-6968.1998.tb13061.x.
Defects in the uvsI gene of Aspergillus nidulans resulted in high UV sensitivity and reductions of spontaneous and UV-induced reversion of certain alleles, uvsl;uvsA double mutants exhibited high methyl methane sulfonate (MMS)-sensitivity in contrast to the slight sensitivity of the component single mutants. Using such a double mutant as recipient, a clone complementing uvsI501 has been isolated from a chromosome III specific library. The deduced amino acid sequence from the 1.1-kb sequenced region, a part of the 5.2-kb DNA fragment showing uvsI-complementing activity, had a 62% identity with REV3 of yeast. Disruptants of the cloned gene demonstrated the same level of sensitivity to UV light as uvsI and failed to complement uvsI501 in heterozygous diploids.
构巢曲霉uvsI基因的缺陷导致其对紫外线高度敏感，某些等位基因的自发回复突变率和紫外线诱导的回复突变率降低，uvsI;uvsA双突变体表现出对甲磺酸甲酯（MMS）高度敏感，而单个组成突变体仅有轻微敏感。以这样的双突变体作为受体，从III号染色体特异性文库中分离出了一个能互补uvsI501的克隆。从显示uvsI互补活性的5.2-kb DNA片段的一部分1.1-kb测序区域推导的氨基酸序列与酵母的REV3有62%的同一性。克隆基因的破坏体对紫外线的敏感程度与uvsI相同，并且在杂合二倍体中不能互补uvsI501。
