Sewell D L, Potter S A, Jacobson C M, Strausbaugh L J, Ward T T
Laboratory Service, Veterans Affairs Medical Center, Portland, OR 97201.
Diagn Microbiol Infect Dis. 1993 Jul;17(1):53-6. doi: 10.1016/0732-8893(93)90070-n.
This study compared the sensitivity of nasal culture alone versus multiple-site cultures and single versus duplicate sampling for the detection of methicillin-resistant Staphylococcus aureus (MRSA)-colonized individuals in a nursing-home population. Repeat culture of 68 specimens collected from 35 colonized subjects yielded identical results for 57 specimens, (84%), and 89% of the colonized residents (31 of 35) were identified by the first culture of multiple sites. A single nares culture detected 27 (77%) of 35 (first screen) and 29 (83%) of 35 (second screen) residents colonized with MRSA at any site. The most cost-effective screening would consist of a nasal culture only or combined with a gastrostomy tube site, if applicable. To identify all colonized individuals, however, it would be necessary to culture more than one specimen from multiple sites on each resident.
本研究比较了单纯鼻腔培养与多部位培养以及单次采样与重复采样在检测疗养院人群中耐甲氧西林金黄色葡萄球菌（MRSA）定植个体方面的敏感性。从35名定植受试者采集的68份标本进行重复培养，57份标本（84%）得到相同结果，89%的定植居民（35名中的31名）通过多部位首次培养得以识别。单次鼻腔培养检测出35名（首次筛查）中27名（77%）以及35名（第二次筛查）中29名（83%）在任何部位定植有MRSA的居民。最具成本效益的筛查方法是仅进行鼻腔培养，或在适用时结合胃造瘘管部位培养。然而，为了识别所有定植个体，有必要对每位居民的多个部位采集不止一份标本进行培养。
