Lightsey A L, Chapman R M, McMillan R, Mushovic J, Yelenosky R, Longmire R L
Ann Intern Med. 1977 Jan;86(1):60-2. doi: 10.7326/0003-4819-86-1-60.
A patient with hyperthyroidism and severe neutropenia had a clinical course and family history that suggested an immune cause. Neutrophil-binding IgG was demonstrated in serum using the Fab-anti Fab assay. The antineutrophil factor bound specifically to either homologous or autologous neutrophils and could be adsorbed by the target neutrophils. The quantity of IgG required to saturate the neutrophil-binding sites (175 000 molecules per neutrophil) and the serum concentration (1.3 mug/ml) were determined. It was estimated that at the time that blood and marrow neutrophils were markedly reduced, serum contained sufficient neutrophil-binding IgG to saturate the binding sites of 1.2 times the total blood neutrophil pool.
一名患有甲状腺功能亢进和严重中性粒细胞减少症的患者，其临床病程和家族史提示存在免疫方面的病因。采用Fab-抗Fab检测法在血清中检测到了与中性粒细胞结合的IgG。抗中性粒细胞因子能特异性地与同源或自身中性粒细胞结合，并可被靶中性粒细胞吸附。测定了使中性粒细胞结合位点饱和所需的IgG量（每中性粒细胞175000个分子）以及血清浓度（1.3μg/ml）。据估计，在血液和骨髓中性粒细胞显著减少时，血清中所含的与中性粒细胞结合的IgG足以使总血液中性粒细胞池结合位点饱和1.2倍。
