Bladt F, Birchmeier C
Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft Carl-von-Linne-Weg, Köln, Germany.
Differentiation. 1993 Jun;53(2):115-22. doi: 10.1111/j.1432-0436.1993.tb00651.x.
We have characterized a murine protein kinase gene, rck, which was identified by crosshybridization with sequences from the v-ros tyrosine kinase gene under conditions of reduced stringency. cDNA analysis indicated that rck encodes a putative protein kinase related to the cdc2 subclass of the gene family and that the gene is identical to mak identified previously in the rat. An extensive expression analysis in the mouse performed by a combination of in situ hybridization and RNase protection revealed a novel and restricted pattern of expression: rck transcripts are found in two cell types involved in sensory transduction, photoreceptors and olfactory receptors as well as in epithelia of the respiratory tract and choroid plexus. Specific transcripts are also found in pre- and postmeiotic male germ cells. We suggest therefore that rck participates in signalling pathways important in a distinct set of cells, remarkably among them cells involved in sensory signal transduction.
我们已对一个小鼠蛋白激酶基因rck进行了特征分析，该基因是在低严谨度条件下通过与v-ros酪氨酸激酶基因的序列交叉杂交而鉴定出来的。cDNA分析表明，rck编码一种与该基因家族的cdc2亚类相关的假定蛋白激酶，并且该基因与先前在大鼠中鉴定出的mak相同。通过原位杂交和核糖核酸酶保护相结合的方法在小鼠中进行的广泛表达分析揭示了一种新的、受限的表达模式：rck转录本存在于参与感觉转导的两种细胞类型中，即光感受器和嗅觉感受器，以及呼吸道和脉络丛的上皮细胞中。在减数分裂前和减数分裂后的雄性生殖细胞中也发现了特异性转录本。因此，我们认为rck参与了一组独特细胞中重要的信号通路，其中显著的是参与感觉信号转导的细胞。
