Drysdale M R, Kolze S E, Kelly J M
Department of Genetics, University of Adelaide, Australia.
Gene. 1993 Aug 25;130(2):241-5. doi: 10.1016/0378-1119(93)90425-3.
In order to undertake a comparative analysis of carbon catabolite repression in two Aspergillus species, the creA gene has been isolated from A. niger by cross hybridization, using the cloned A. nidulans gene. The A. niger gene has been shown to be functional in A. nidulans by heterologous complementation of the creA204 mutation of A. nidulans. Overall, the genes show 90% sequence similarity (82% identity) at the amino acid (aa) level. There were some striking similarities between the aa sequences encoded by the two fungal creA genes and two genes involved in carbon catabolite repression in Saccharomyces cerevisiae. The zinc-finger regions showed 96% similarity (84% identity) with the zinc-finger region of the MIG1 gene of S. cerevisiae. The CREA protein contains a stretch of 42 aa that is identical in A. niger and A. nidulans, and these show 81% similarity (33% identity) with a region of the S. cerevisiae RGR1 gene.
为了对两种曲霉属物种中的碳代谢物阻遏进行比较分析，利用克隆的构巢曲霉基因通过交叉杂交从黑曲霉中分离出creA基因。通过对构巢曲霉creA204突变体的异源互补，已证明黑曲霉基因在构巢曲霉中具有功能。总体而言，这些基因在氨基酸水平上显示出90%的序列相似性（82%的同一性）。两种真菌creA基因编码的氨基酸序列与酿酒酵母中参与碳代谢物阻遏的两个基因之间存在一些显著的相似性。锌指区域与酿酒酵母MIG1基因的锌指区域显示出96%的相似性（84%的同一性）。CREA蛋白包含一段42个氨基酸的序列，在黑曲霉和构巢曲霉中是相同的，并且这些序列与酿酒酵母RGR1基因的一个区域显示出81%的相似性（33%的同一性）。
