MacCabe A P, Vanhanen S, Sollewign Gelpke M D, van de Vondervoort P J, Arst H N, Visser J
Section Molecular Genetics of Industrial Microorganisms, Agricultural University, Wageningen, Netherlands.
Biochim Biophys Acta. 1998 Mar 9;1396(2):163-8. doi: 10.1016/s0167-4781(97)00212-1.
The gene encoding the positive-acting regulator of nitrogen metabolite repression (AREA) has been cloned and characterised from the industrially important filamentous fungus Aspergillus niger. The deduced amino acid sequence has an overall level of identity with its homologues from other fungal species which varies between 32 and 72%. This gene (areAnig) complements the A. nidulans areAr-18 loss-of-function mutation. Sequences upstream of the structural gene contain several putative GATA-type zinc finger protein-binding motifs. Northern analysis indicates the synthesis of multiple transcripts, the major species being approximately 2.95 kb and 3.1 kb. Maximal expression of areAnig is observed under conditions of nitrogen starvation and is mainly due to an increase in the level of the shorter transcript.
