Dyck L E
Department of Psychiatry, University of Saskatchewan, Saskatoon, Canada.
Alcohol Clin Exp Res. 1990 Aug;14(4):534-8. doi: 10.1111/j.1530-0277.1990.tb01195.x.
The types of isozymes of aldehyde dehydrogenase (ALDH) present in human lymphocytes has been investigated using isoelectric focusing of polyacrylamide gels followed by substrate-specific staining. Lymphocytes obtained from most individuals were found to contain both types I and II ALDH. This group of 'typical' individuals reported that they did not develop marked facial flushing or rapid heart rate after drinking alcohol nor did they develop an erythema to cutaneously applied ethanol. Lymphocytes obtained from 'atypical' individuals who do suffer from alcohol-induced flushing and rapid heart rate and who developed erythema to cutaneous ethanol displayed type II, but not type I, ALDH. Lymphocytes thus appear to be an easily accessible and suitable tissue for determining type I ALDH phenotype.
利用聚丙烯酰胺凝胶等电聚焦，继以底物特异性染色，对人淋巴细胞中存在的醛脱氢酶（ALDH）同工酶类型进行了研究。发现从大多数个体获取的淋巴细胞同时含有I型和II型ALDH。这群“典型”个体报告称，他们饮酒后不会出现明显的面部潮红或心率加快，皮肤涂抹乙醇后也不会出现红斑。从“非典型”个体获取的淋巴细胞，这些个体确实会出现酒精引起的潮红和心率加快，且皮肤涂抹乙醇后会出现红斑，这些淋巴细胞显示有II型而非I型ALDH。因此，淋巴细胞似乎是一种易于获取且适合用于确定I型ALDH表型的组织。
