以流产布鲁氏菌分离为标准，比较颗粒浓度荧光免疫测定法与卡片试验和补体结合试验。
Comparison of particle concentration fluorescence immunoassay to card and complement fixation tests using isolation of Brucella abortus as the standard.
作者信息
Greenlee M T, Farrar J A, Hird D W, Holmes J C
机构信息
US Department of Agriculture, Animal and Plant Health Inspection Service, Arlington, TX 76010.
出版信息
J Vet Diagn Invest. 1994 Apr;6(2):182-7. doi: 10.1177/104063879400600208.
Serologic test data and bacteriologic culture results from 816 cattle were evaluated. Brucella abortus (field strain or strain 19) was isolated from 27.3% of the cows. Results of the card test, particle concentration fluorescence immunoassay (PCFIA), and complement fixation (CF) test were compared. Antibody titers were directly associated with B. abortus isolation, with the majority (64.7%) of field strain isolations having a PCFIA value of < or = 0.300 and a CF test result of at least 1+ at a 1:160 dilution. The specificity of the CF test was significantly higher than that of the PCFIA (at CF > or = 1:40 and PCFIA < or = 0.300).
对816头牛的血清学检测数据和细菌培养结果进行了评估。从27.3%的奶牛中分离出了流产布鲁氏菌（野毒株或19号菌株）。比较了卡片试验、颗粒凝集荧光免疫测定（PCFIA）和补体结合（CF）试验的结果。抗体滴度与流产布鲁氏菌的分离直接相关，大多数（64.7%）野毒株分离株的PCFIA值≤0.300，且在1:160稀释度下CF试验结果至少为1+。CF试验的特异性显著高于PCFIA（CF≥1:40且PCFIA≤0.300）。
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