Kumagai K, Fukui A, Tanaka S, Ikemoto M, Moriguchi K, Nabeshima S
Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co., Ltd., Hyogo, Japan.
J Antibiot (Tokyo). 1993 Jul;46(7):1139-44. doi: 10.7164/antibiotics.46.1139.
A new macrolide antibiotic, PC-766B, was isolated from the cells of Nocardia brasiliensis SC-4710 by acetone extraction, and purified by gel filtration, silica gel chromatography, HPLC and TLC. The structure of PC-766B was determined by NMR spectral analysis to be a new class of the hygrolidin family antibiotics. PC-766B had a 16-membered macrocyclic lactone ring, a 6-membered hemiketal ring and a 2-deoxy-D-rhamnose moiety. DL-alpha-Tocopherol, known as an antioxidant agent, significantly improved the stability of PC-766B and prevented the decomposition of PC-766B during the storage of the antibiotic.
一种新的大环内酯类抗生素PC-766B，通过丙酮萃取从巴西诺卡氏菌SC-4710的细胞中分离出来，并通过凝胶过滤、硅胶柱色谱、高效液相色谱和薄层色谱进行纯化。通过核磁共振光谱分析确定PC-766B的结构为新型的潮霉素家族抗生素。PC-766B具有一个16元大环内酯环、一个6元半缩酮环和一个2-脱氧-D-鼠李糖部分。DL-α-生育酚，即众所周知的抗氧化剂，可显著提高PC-766B的稳定性，并防止该抗生素在储存过程中分解。
