Nakata T, Takemura R, Hirokawa N
Department of Anatomy and Cell Biology, University of Tokyo, School of Medicine, Japan.
J Cell Sci. 1993 May;105 ( Pt 1):1-5. doi: 10.1242/jcs.105.1.1.
Dynamin is a member of a new GTPase family, which includes the mouse Mx protein, the yeast VPS1 and the Drosophila shibire gene product. A high homology with the shibire product suggests a role for dynamin in the endocytotic process, but it is expressed only in mature neurons. We identified two additional dynamin-like proteins in rats, by using the polymerase chain reaction with degenerate primers corresponding to the GTP-binding areas conserved between dynamin and VPS1. The full coding sequence of one of them, dynamin-2, revealed that it has 848 amino acids and has great similarity with brain dynamin and the shibire product. Northern blot analysis and in situ hybridization revealed its expression to be specific to the seminiferous tubules in the testis. Dynamin-2 (testis type dynamin) was expressed in germ-cell-depleted testis as well, indicating its expression in Sertoli cells. Our data imply that a number of dynamin family proteins, which are products of distinct genes, may play different roles specific to each cell type in the same rat.
发动蛋白是一个新的GTP酶家族的成员，该家族包括小鼠Mx蛋白、酵母VPS1和果蝇的发动蛋白基因产物。与发动蛋白基因产物的高度同源性表明发动蛋白在胞吞过程中发挥作用，但它仅在成熟神经元中表达。我们通过使用与发动蛋白和VPS1之间保守的GTP结合区域相对应的简并引物进行聚合酶链反应，在大鼠中鉴定出另外两种类似发动蛋白的蛋白质。其中一种发动蛋白-2的完整编码序列显示，它有848个氨基酸，与脑发动蛋白和发动蛋白基因产物有很大的相似性。Northern印迹分析和原位杂交显示其表达仅限于睾丸的生精小管。发动蛋白-2（睾丸型发动蛋白）在生精细胞缺失的睾丸中也有表达，表明它在支持细胞中表达。我们的数据表明，许多发动蛋白家族蛋白是不同基因的产物，可能在同一只大鼠的每种细胞类型中发挥不同的特定作用。
