Darby J M, Nemoto E M, Yonas H, Yao L, Melick J A, Boston J R
Department of Anesthesiology and Critical Care Medicine, Presbyterian University Hospital, Pittsburgh, Pennsylvania 15261.
J Cereb Blood Flow Metab. 1993 Sep;13(5):763-72. doi: 10.1038/jcbfm.1993.97.
We developed a closed-skull model of freeze injury-induced brain edema, a model classically thought to produce vasogenic edema, and observed the natural course of changes in edema and blood flow using xenon-enhanced computed tomography (CT) in five rhesus monkeys before and for up to 6 h post insult. Intracranial pressure (ICP) gradually rose throughout the duration of the experiment. CT scans and CBF images permitted direct observation of the evolution of the lesion and revealed early ischemia in the periphery of the injury zone that progressed over time in association with edema. Frequency histogram analysis of local CBF (ICBF) demonstrated subtle but potentially important changes in distribution of ICBF between and within hemispheres at various times post insult. Changes in ICBF distribution were phasic and dissociated from increases in ICP in the latter stages of injury. The Xe/CT CBF method can be used to evaluate the effects of injury and therapy on CBF in this and other models of acute brain injury.
我们建立了一个冷冻损伤诱导脑水肿的闭合性颅骨模型，该模型传统上被认为会产生血管源性水肿，并使用氙增强计算机断层扫描（CT）观察了5只恒河猴在损伤前及损伤后长达6小时内水肿和血流变化的自然过程。在整个实验过程中，颅内压（ICP）逐渐升高。CT扫描和脑血流量（CBF）图像可以直接观察到病变的演变，并显示出损伤区域周边早期出现的缺血，随着时间的推移，缺血与水肿相关联。局部脑血流量（ICBF）的频率直方图分析表明，在损伤后的不同时间，半球间和半球内ICBF分布存在细微但可能重要的变化。在损伤后期，ICBF分布的变化呈阶段性，且与ICP的升高无关。氙/CT脑血流量方法可用于评估该模型及其他急性脑损伤模型中损伤和治疗对脑血流量的影响。
