Di Muccio A, Dommarco R, Attard Barbini D, Santilio A, Girolimetti S, Ausili A, Ventriglia M, Generali T, Vergori L
Laboratorio di Tossicologia Applicata, Istituto Superiore di Sanità, Rome, Italy.
J Chromatogr. 1993 Jul 23;643(1-2):363-8. doi: 10.1016/0021-9673(93)80572-p.
Disposable, ready-to-use cartridges filled with a macroporous diatomaceous material are used to extract in a single step fungicide residues with dichloromethane from aqueous acetone extracts of vegetables. This procedure takes the place of some functions (such as separating funnel partition, drying over anhydrous sodium sulphate and clean-up) usually performed by separate steps in classical schemes. Fourteen fungicides (dichloran, vinclozolin, chlorthalonil, triadimefon, dichlofluanide, procymidone, hexaconazole, captan, folpet, ditalimfos, iprodione, captafol, pyrazophos and fenarimol) were determined using the described procedure with recoveries between 83 and 107% at spiking levels ranging for the different compounds from 0.04 to 0.40 mg/kg. Crops subjected to the described procedure included lettuce, strawberry, apple, yellow pepper and peach, and gave extracts containing a mass of co-extractives between 5 and 30 mg. Compared with classical schemes, the described procedure is simple, less labour intensive, allows parallel handling of several extracts and does not require preparation and maintenance of equipment. Troublesome emulsions such as those frequently observed in separating funnel partitioning do not occur.
一次性即用型小柱填充有大孔硅藻土材料，用于从蔬菜的丙酮水溶液提取物中一步提取二氯甲烷中的杀菌剂残留。该方法替代了传统方法中通常分几步进行的一些操作（如分液漏斗分配、无水硫酸钠干燥和净化）。使用所述方法测定了14种杀菌剂（百菌清、乙烯菌核利、百菌清、三唑酮、敌菌灵、腐霉利、己唑醇、克菌丹、灭菌丹、异稻瘟净、异菌脲、克普丹、吡唑硫磷和氯苯嘧啶醇），在不同化合物的加标水平为0.04至0.40mg/kg时，回收率在83%至107%之间。采用所述方法处理的作物包括生菜、草莓、苹果、黄椒和桃子，提取物中含有5至30mg的共提取物。与传统方法相比，所述方法简单，劳动强度小，允许同时处理多个提取物，并且不需要设备的准备和维护。不会出现分液漏斗分配中经常出现的麻烦乳液。
