Jaffar-Bandjee M C, Carrere J, Lazdunski A, Guy-Crotte O, Galabert C
CERM, Hôpital Renée Sabran, Giens, Hyères, France.
J Immunol Methods. 1993 Aug 26;164(1):27-32. doi: 10.1016/0022-1759(93)90272-9.
A direct sandwich enzyme immunoassay was developed in order to quantify Pseudomonas aeruginosa elastase. As a solid phase the wells of a microtitre plate were coated with specific IgG and horseradish peroxidase labelled IgG was used as the second antibody. The detection limit of the assay was 0.26 ng/ml and a good agreement was found with elastolytic activity determined using elastin-Congo red. This assay was simple, specific, sensitive and reproducible, and permits the determination of low levels of elastase.
为了定量检测铜绿假单胞菌弹性蛋白酶，开发了一种直接夹心酶免疫测定法。以微量滴定板的孔作为固相，用特异性IgG包被，辣根过氧化物酶标记的IgG用作第二抗体。该测定法的检测限为0.26 ng/ml，并且与使用弹性蛋白-刚果红测定的弹性水解活性具有良好的一致性。该测定法简单、特异、灵敏且可重复，能够测定低水平的弹性蛋白酶。
