Döring G, Obernesser H J, Botzenhart K, Flehmig B, Høiby N, Hofmann A
J Infect Dis. 1983 Apr;147(4):744-50. doi: 10.1093/infdis/147.4.744.
Radioimmunoassays were used to determine titers of antibody to alkaline protease (AP) and elastase (Ela) produced by Pseudomonas aeruginosa in sera and bronchial secretions, in vitro production of AP and Ela by P. aeruginosa isolates, and occurrence of these enzymes in bronchial secretions from patients with cystic fibrosis. Titers of serum antibodies to AP ranging from 1:10 to 1:545 and to Ela ranging from 1:10 to 1:725 were found in 83%-88% of patients with cystic fibrosis and chronic lung infections due to P. aeruginosa. Antibody titers in liquified bronchial secretions were approximately 10% of the serum titers. Thirty-one (93%) of 34 isolates produced both proteases in vitro in comparable amounts (concentration of protease in culture supernatant: AP, 0.01-480 micrograms/ml; Ela, 0.02-490 micrograms/ml). AP and Ela were detected in vivo when antibodies to the enzymes were absent. The results suggest that specific immune responses in patients with cystic fibrosis neutralize proteases of P. aeruginosa.
采用放射免疫分析法测定血清和支气管分泌物中针对铜绿假单胞菌产生的碱性蛋白酶（AP）和弹性蛋白酶（Ela）的抗体滴度、铜绿假单胞菌分离株体外产生AP和Ela的情况，以及囊性纤维化患者支气管分泌物中这些酶的存在情况。在83%-88%的囊性纤维化患者和因铜绿假单胞菌引起慢性肺部感染的患者中，发现血清中针对AP的抗体滴度范围为1:10至1:545，针对Ela的抗体滴度范围为1:10至1:725。液化支气管分泌物中的抗体滴度约为血清滴度的10%。34株分离株中有31株（93%）在体外产生这两种蛋白酶的量相当（培养上清液中蛋白酶浓度：AP为0.01-480微克/毫升；Ela为0.02-490微克/毫升）。在体内未检测到针对这些酶的抗体时，检测到了AP和Ela。结果表明，囊性纤维化患者的特异性免疫反应可中和铜绿假单胞菌的蛋白酶。
