Hiatt A, Ma J K
Department of Cell Biology, Scripps Research Institute, La Jolla, California.
Int Rev Immunol. 1993;10(2-3):139-52. doi: 10.3109/08830189309061691.
Of the variety of compounds expressed in transgenic plants, antibodies offer probably the widest range of applications. The antibodies appear to possess all of the functional characteristics of antibody derived from hybridoma cells, although further study will be required to determine the effect of the difference in heavy chain glycosylation. More work will also be directed toward the assembly, accumulation, stability and secretion of plant antibodies. The effect of the signal sequence on the expression and assembly of antibodies has been shown and further methods of optimizing transgenic protein accumulation in plants almost certainly exist. Plant antibody technology is still in its infancy. However, it offers enormous potential in "mix-and-match" antibody engineering, and the construction of multimeric immunoglobulin complexes may be feasible relatively easily, for the first time. Furthermore, as there is an enduring interest in using antibodies for therapeutic purposes, agricultural production and distribution offers a means of obtaining large quantities of antibodies at a relatively low cost.
在转基因植物中表达的各种化合物中，抗体的应用范围可能最为广泛。这些抗体似乎具备源自杂交瘤细胞的抗体的所有功能特性，不过还需要进一步研究来确定重链糖基化差异的影响。更多的工作也将针对植物抗体的组装、积累、稳定性和分泌。信号序列对抗体表达和组装的影响已得到证实，几乎可以肯定还存在进一步优化转基因蛋白在植物中积累的方法。植物抗体技术仍处于起步阶段。然而，它在“混合搭配”抗体工程中具有巨大潜力，而且首次相对容易地构建多聚体免疫球蛋白复合物可能是可行的。此外，由于人们一直对将抗体用于治疗目的感兴趣，农业生产和分销提供了一种以相对低成本获取大量抗体的途径。
