Yamashita U, Tanaka Y, Shirakawa F
Department of Immunology and Parasitology, Hiroshima University, School of Medicine, Japan.
J Leukoc Biol. 1993 Aug;54(2):133-7. doi: 10.1002/jlb.54.2.133.
The effect of interleukin-4 (IL-4) on the proliferation and differentiation of myeloid leukemic cell lines was studied in vitro. A culture of murine myeloid cell line, M1, with lipopolysaccharide (LPS) from Escherichia coli, induced differentiation into macrophages that expressed Fc receptors and phagocytic activity. IL-4 did not induce the differentiation of M1 cells but inhibited the differentiation of M1 cells induced with LPS. On the other hand, LPS arrested the proliferation of M1 cells. IL-4 had no effect on the proliferation of M1 cells but restored the LPS-induced arrest of the proliferation of M1 cells. IL-1, IL-6 and tumor necrosis factor alpha (TNF-alpha) also induced the differentiation of M1 cells into macrophages and arrested proliferation. IL-4 suppressed the IL-1-, IL-6-, and TNF-induced differentiation of M1 cells and restored the arrested proliferation with IL-1, IL-6, and TNF. Similar results were obtained with human myeloid cell line HL60. These results suggest that IL-4 has a suppressive effect on the differentiation of myeloid cells into macrophages.
在体外研究了白细胞介素-4（IL-4）对髓系白血病细胞系增殖和分化的影响。用来自大肠杆菌的脂多糖（LPS）培养小鼠髓系细胞系M1，可诱导其分化为表达Fc受体和具有吞噬活性的巨噬细胞。IL-4未诱导M1细胞分化，但抑制了LPS诱导的M1细胞分化。另一方面，LPS阻止了M1细胞的增殖。IL-4对M1细胞的增殖没有影响，但恢复了LPS诱导的M1细胞增殖停滞。白细胞介素-1（IL-1）、白细胞介素-6（IL-6）和肿瘤坏死因子α（TNF-α）也诱导M1细胞分化为巨噬细胞并阻止其增殖。IL-4抑制了IL-1、IL-6和TNF诱导的M1细胞分化，并用IL-1、IL-6和TNF恢复了停滞的增殖。人髓系细胞系HL60也得到了类似结果。这些结果表明，IL-4对髓系细胞分化为巨噬细胞具有抑制作用。
