Boyd E F, Wang F S, Beltran P, Plock S A, Nelson K, Selander R K
Institute of Molecular Evolutionary Genetics, Mueller Laboratory, Pennsylvania State University, Pennsylvania 16802.
J Gen Microbiol. 1993 Jun;139 Pt 6:1125-32. doi: 10.1099/00221287-139-6-1125.
A reference collection of 72 strains representing 37 serovars of Salmonella subspecies I has been established for use in research on genetic and phenotypic variation in natural populations. Included are isolates of the host-adapted serovars S. choleraesuis, S. dublin, S. gallinarum, S. paratyphi A, S. paratyphi B, S. paratyphi C, S. pullorum, S. sendai, S. typhi and S. typhisuis, as well as strains of S. enteritidis, S. typhimurium, and other commonly recovered serovars with broad host ranges. The isolates were characterized by enzyme electrophoresis for allelic variation in 25 chromosomal genes and represent 71 distinctive multilocus genotypes (electrophoretic types or ETs). Genetic relationships among the ETs are indicated in an evolutionary tree constructed by the neighbour-joining method from a matrix of Nei's standard genetic distance.
已建立了一个参考菌株库,包含72个菌株,代表沙门氏菌亚种I的37个血清型,用于自然种群遗传和表型变异的研究。其中包括宿主适应性血清型猪霍乱沙门氏菌、都柏林沙门氏菌、鸡沙门氏菌、甲型副伤寒沙门氏菌、乙型副伤寒沙门氏菌、丙型副伤寒沙门氏菌、鸡白痢沙门氏菌、仙台沙门氏菌、伤寒沙门氏菌和猪伤寒沙门氏菌的分离株,以及肠炎沙门氏菌、鼠伤寒沙门氏菌和其他常见的具有广泛宿主范围的血清型菌株。通过酶电泳对这些分离株的25个染色体基因的等位基因变异进行了表征,它们代表了71种独特的多位点基因型(电泳类型或ETs)。ETs之间的遗传关系在通过邻接法从Nei标准遗传距离矩阵构建的进化树中显示。