Krivko I M, Rusakov D A, Savina S V, Skibo G G, Berezin V A
Dniepropetrovsk State University, Ukraine.
Neurosci Lett. 1993 May 14;154(1-2):17-9. doi: 10.1016/0304-3940(93)90160-m.
In monolayer cultures of newborn rat hippocampal cells, immunogold-labelling at the electron microscope level was used to study quantitatively the neural cell adhesion molecule (N-CAM) arrangement on the surface of glial soma and processes on 5 and 12 days in vitro (DIV). Four corresponding samples of micrographs were formed. To quantify the labelling, a stochastic geometry approach was used. Spectra of lateral distances between labels as well as simulated images of the surface label arrangement (invisible in micrographs) were derived and compared. The data show that, on both 5 and 12 DIV, N-CAM density on the surface of processes is approximately 2 times higher than that in somata; 12-DIV cells showing a lower (approximately 25%) N-CAM surface density as compared with the 5-DIV cells. This suggests that N-CAM expression in glia surfaces decreases while the cells form contacts, and N-CAM sorting between soma and processes remains stable. The simulated topographies of the lateral N-CAM arrangement might highlight fundamental mechanisms that underlie formation of the neural network.
在新生大鼠海马细胞的单层培养中，利用电子显微镜水平的免疫金标记技术，对体外培养5天和12天（DIV）时神经胶质细胞体和突起表面的神经细胞黏附分子（N-CAM）排列进行了定量研究。形成了四个相应的显微照片样本。为了对标记进行定量，采用了随机几何方法。得出了标记之间横向距离的光谱以及表面标记排列的模拟图像（显微照片中不可见）并进行了比较。数据表明，在培养5天和12天时，突起表面的N-CAM密度大约是细胞体中的2倍；与培养5天的细胞相比，培养12天的细胞显示出较低（约25%）的N-CAM表面密度。这表明，在神经胶质细胞形成接触时，其表面的N-CAM表达减少，并且细胞体和突起之间的N-CAM分选保持稳定。横向N-CAM排列的模拟拓扑结构可能突出了神经网络形成的基本机制。
