Rogard M, Caboche J, Julien J F, Besson M J
Laboratoire de Neurochimie-Anatomie, Institut des Neurosciences, URA 1488, Université P. et M. Curie, Paris, France.
Neurosci Lett. 1993 May 28;155(1):81-6. doi: 10.1016/0304-3940(93)90678-e.
The distribution of messenger RNAs (mRNAs) encoding the 67 kDa isoform of glutamic acid decarboxylase (GAD67), a rate-limiting enzyme in the biosynthesis of gamma-aminobutyric acid, and preproenkephalin (PPE), the precursor of enkephalin, was analysed in the rat nucleus accumbens (NAS) taking in account its compartmentation in 'core' (NAS(core)) and 'shell' (NASshell). While GAD67 mRNA levels were more abundant (+48%) in NASshell than in NAS(core), hybridization signals for PPE mRNA were mainly found in NAScore. At a cellular level, hybridization signals for GAD67 mRNA were present in 75% and 80% of neurons NASshell and NAS(core), respectively. The level of GAD67 mRNA found higher in the NASshell corresponds in fact to a greater density of neurons in NASshell than in the NAS(core). The hybridization signals for PPE mRNA were observed in very few neurons of NASshell and in around half of the neurons in NAS(core). Besides this medio to lateral difference, GAD67 and PPE mRNAs were distributed in NAS(core) according to a rostro-caudal gradient, the content of both mRNAs being higher in the rostral part than the caudal part of this area.
考虑到伏隔核（NAS）分为“核心”（NAS（core））和“壳”（NASshell）两个部分，我们分析了编码γ-氨基丁酸生物合成限速酶——谷氨酸脱羧酶67 kDa亚型（GAD67）的信使核糖核酸（mRNA）以及脑啡肽前体——前脑啡肽原（PPE）在大鼠伏隔核中的分布情况。虽然GAD67 mRNA水平在NASshell中比在NAS（core）中更为丰富（+48%），但PPE mRNA的杂交信号主要出现在NAScore中。在细胞水平上，GAD67 mRNA的杂交信号分别出现在NASshell和NAS（core）中75%和80%的神经元中。实际上，在NASshell中发现的较高GAD67 mRNA水平对应于NASshell中比NAS（core）中更高的神经元密度。PPE mRNA的杂交信号在NASshell的极少数神经元中以及NAS（core）中约一半的神经元中被观察到。除了这种内侧到外侧的差异外，GAD67和PPE mRNAs在NAS（core）中按照头尾梯度分布，该区域头侧部分这两种mRNA的含量均高于尾侧部分。
