大鼠前脑产前D1、D1b和D3多巴胺受体基因表达：通过逆转录聚合酶链反应进行检测

Prenatal D1, D1b and D3 dopamine receptor gene expression in the rat forebrain: detection by reverse polymerase chain reaction.

作者信息

Cadoret M A, Jaber M, Bloch B

机构信息

URA CNRS 1200, Laboratoire d'Histologie-Embryologie, UFR II, Université de Bordeaux II, France.

出版信息

Neurosci Lett. 1993 May 28;155(1):92-5. doi: 10.1016/0304-3940(93)90680-j.

DOI:10.1016/0304-3940(93)90680-j

PMID:8361670

Abstract

Three dopamine receptor mRNAs were detected by reverse polymerase chain reaction in the rat forebrain during ontogeny. The D1 receptor mRNA gave a faint signal from gestational day 11 (G11) on, while the D1b receptor was not detectable before G12. The D3 receptor mRNA was slightly expressed at G11 and clearly present at G14. Our results show that these mRNAs are present at early stages of fetal life, each one with its specific time of appearance.

摘要

在个体发育过程中，通过逆转录聚合酶链反应在大鼠前脑中检测到三种多巴胺受体mRNA。从妊娠第11天（G11）开始，D1受体mRNA发出微弱信号，而在G12之前未检测到D1b受体。D3受体mRNA在G11时略有表达，在G14时明显存在。我们的结果表明，这些mRNA在胎儿生命的早期阶段就已存在，每种mRNA都有其特定的出现时间。