Nagai Y, Ueno S, Saeki Y, Soga F, Hirano M, Yanagihara T
Department of Neurology, Osaka University Medical School, Osaka, Japan.
Neurology. 1996 Mar;46(3):791-5. doi: 10.1212/wnl.46.3.791.
We investigated the dopamine receptor (DAR) mRNA expression in peripheral blood lymphocytes from 45 patients with Parkinson's disease (PD) and 21 age-matched controls using the quantitative reverse transcription and polymerase chain reaction method. Beta-actin mRNA was used as an internal control to evaluate the relative expression level of the DAR mRNA. There was a statistically significant decrease of the D3 dopamine receptor (D3R) mRNA expression in PD patients compared with that in controls. There was no change in expression of the D5 dopamine reception mRNA in PD patients. A further binding study showed reduction of the D3R binding sites in PD lymphocytes. The decrease of the D3R mRNA expression correlated with the degree of clinical severity in PD patients.
我们采用定量逆转录聚合酶链反应方法，研究了45例帕金森病（PD）患者及21例年龄匹配的对照者外周血淋巴细胞中多巴胺受体（DAR）mRNA的表达情况。以β-肌动蛋白mRNA作为内对照，评估DAR mRNA的相对表达水平。与对照组相比，PD患者中D3多巴胺受体（D3R）mRNA表达有统计学意义的降低。PD患者中D5多巴胺受体mRNA的表达无变化。进一步的结合研究显示，PD淋巴细胞中D3R结合位点减少。D3R mRNA表达的降低与PD患者的临床严重程度相关。
