[The in-vitro digestive availability of lipase and trypsin in pancreatic supplements under different degrees of acidity].

We evaluated the in vitro disintegration time and the remanent digestive activity of eight pancreatic supplements under pH conditions similar to the gastrointestinal tract. They were incubated for 45 min at various pH levels (1, 3 or 6) and continued thereafter at pH 6, for another 135 min. The activities of lipase and trypsin were evaluated titrimetrically every 15 min. At pH 6, the products without an enteric coat and Creon, showed the shortest disintegration times; under acidic conditions, those times were longer in all the enteric coated products. At constant pH 6, lipase activity was greater in Creon, Pankreon and Cotazym-B; trypsin activity was greater in Nutrizym-C, Onoton and Cotazym-B. After acidic pH exposure enzyme bioavailability was decreased in all the products. Disintegration times and acid inactivation of enzymes, should be considered when prescribing PS.