Acevedo A, del Pozo M A, Arroyo A G, Sánchez-Mateos P, González-Amaro R, Sánchez-Madrid F
Department of Pathology, Hospital de la Princesa, Universidad Autónoma de Madrid, Spain.
Am J Pathol. 1993 Sep;143(3):774-83.
The LFA-1 integrin mediates its function in leukocyte intercellular interactions by recognition of at least one of its three identified counter-receptors, intercellular adhesion molecules 1 (ICAM-1), 2, and 3. The ICAM-1 molecule is expressed in an inducible-dependent manner by endothelial and epithelial cells, as well as by other cell types, including leukocytes. On the other hand, ICAM-2 is constitutively expressed mainly by endothelial cells. We have studied the tissue distribution of the ICAM-3 molecule by immunohistochemical staining of lymphoid and nonlymphoid organs. Only cells from the leukocyte lineage were found bearing the ICAM-3 antigen, showing a pattern of expression clearly distinct from those of ICAM-1 and ICAM-2. Interestingly, we have found that ICAM-3 is expressed by epidermal dendritic Langerhans cells as assessed by double immunostaining with antibodies specific for CD1. In contrast, staining of skin sections with anti-ICAM-1 and ICAM-2 antibodies showed an undetectable expression of these two molecules on Langerhans cells. However, CD1+ Langerhans cells localized in the paracortical area of dermatopathic lymph nodes expressed both ICAM-1 and ICAM-3 antigens. Our results indicate that ICAM-3 is the main LFA-1 counter-receptor in human resident epidermal Langerhans cells. ICAM-3 may have an important role in T-cell antigen stimulation driven by Langerhans cells during skin immune reactions.
淋巴细胞功能相关抗原-1（LFA-1）整合素通过识别其三种已确定的反受体中的至少一种，即细胞间黏附分子1（ICAM-1）、2和3，来介导其在白细胞细胞间相互作用中的功能。ICAM-1分子以内皮细胞和上皮细胞以及包括白细胞在内的其他细胞类型诱导依赖性方式表达。另一方面，ICAM-2主要由内皮细胞组成性表达。我们通过对淋巴器官和非淋巴器官进行免疫组织化学染色研究了ICAM-3分子的组织分布。仅发现白细胞谱系的细胞带有ICAM-3抗原，其表达模式明显不同于ICAM-1和ICAM-2。有趣的是，我们发现通过用针对CD1的特异性抗体进行双重免疫染色评估，ICAM-3由表皮树突状朗格汉斯细胞表达。相比之下，用抗ICAM-1和ICAM-2抗体对皮肤切片进行染色显示这两种分子在朗格汉斯细胞上未检测到表达。然而，位于皮肤性淋巴结副皮质区的CD1 +朗格汉斯细胞同时表达ICAM-1和ICAM-3抗原。我们的结果表明，ICAM-3是人类常驻表皮朗格汉斯细胞中主要的LFA-1反受体。ICAM-3可能在皮肤免疫反应期间由朗格汉斯细胞驱动的T细胞抗原刺激中起重要作用。
