鸡毒支原体假定细胞粘附素基因的鉴定及其作为DNA探针的应用。

Identification of the putative cytadhesin gene of Mycoplasma gallisepticum and its use as a DNA probe.

作者信息

Dohms J E, Hnatow L L, Whetzel P, Morgan R, Keeler C L

机构信息

Delaware Agricultural Experiment Station, Department of Animal Science and Agricultural Biochemistry, College of Agricultural Sciences, University of Delaware, Newark 19717-1303.

出版信息

Avian Dis. 1993 Apr-Jun;37(2):380-8.

PMID:8363503

Abstract

A portion of the putative Mycoplasma gallisepticum (MG) cytadhesin gene was identified and used as a diagnostic DNA probe. Degenerate oligonucleotide primers corresponding to conserved regions of the cytadhesin proteins from two human mycoplasmas, M. pneumoniae and M. genitalium, were synthesized for use in the polymerase chain reaction (PCR) on genomic MG DNA. A 583-base-pair MG DNA fragment was amplified and subsequently cloned and sequenced. The MG DNA fragment is predicted to encode a 193-amino-acid peptide. This peptide demonstrates significant homology to the expected portions of the two human mycoplasmal cytadhesin proteins. Used as a probe to study the distribution of this fragment in pathogenic and nonpathogenic avian mycoplasmas, the PCR product hybridized to genomic DNA from all seven MG strains tested. However, it failed to hybridize to M. synoviae, M. meleagridis, M. iowae, or M. gallinarum DNA.

摘要

鉴定出了部分假定的鸡毒支原体（MG）细胞粘附素基因，并将其用作诊断性DNA探针。合成了与两种人支原体（肺炎支原体和生殖支原体）细胞粘附素蛋白保守区域相对应的简并寡核苷酸引物，用于对MG基因组DNA进行聚合酶链反应（PCR）。扩增出一个583个碱基对的MG DNA片段，随后进行克隆和测序。预测该MG DNA片段编码一个193个氨基酸的肽。该肽与两种人支原体细胞粘附素蛋白的预期部分显示出显著的同源性。用作探针研究该片段在致病性和非致病性禽支原体中的分布时，PCR产物与所检测的所有7株MG菌株的基因组DNA杂交。然而，它未能与滑液支原体、火鸡支原体、伊氏支原体或鸡支原体的DNA杂交。