Garcia M, Jackwood M W, Levisohn S, Kleven S H
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602-4875, USA.
Avian Dis. 1995 Jul-Sep;39(3):606-16.
A single set of oligonucleotide primers was designed from known 16S ribosomal RNA (rRNA) sequences of Mycoplasma gallisepticum (MG), M. synoviae (MS), and M. iowae (MI). This set of primers selectively amplifies a 780-base-pair DNA fragment within the 16S rRNA gene of MG, MS, and MI but does not amplify other avian mycoplasmas or other bacteria. The detection limit of the multi-species polymerase chain reaction (PCR) was approximately 100 mycoplasma (MG, MS, MI) colony-forming units per PCR reaction. The PCR product was differentiated by restriction fragment length polymorphism with the restriction enzymes HpaI, HpaII, and MboI. Preliminary results from field samples suggest that this technique could be a useful and rapid diagnostic test for the detection of these three pathogenic poultry mycoplasmas.
根据鸡毒支原体（MG）、滑液支原体（MS）和衣阿华支原体（MI）已知的16S核糖体RNA（rRNA）序列设计了一套寡核苷酸引物。这套引物可选择性扩增MG、MS和MI的16S rRNA基因内一段780个碱基对的DNA片段，但不会扩增其他禽支原体或其他细菌。多物种聚合酶链反应（PCR）的检测限约为每个PCR反应100个支原体（MG、MS、MI）菌落形成单位。用限制性内切酶HpaI、HpaII和MboI通过限制性片段长度多态性对PCR产物进行鉴别。田间样本的初步结果表明，该技术可能是检测这三种致病性家禽支原体的一种有用且快速的诊断测试。
