DiCesare J, Grossman B, Katz E, Picozza E, Ragusa R, Woudenberg T
Perkin-Elmer Corporation, Wilton, CT 06897.
Biotechniques. 1993 Jul;15(1):152-7.
A high-sensitivity nonisotopic system has been developed for post-PCR product detection. The probe-based detection system exploits a chemiluminescent reaction that takes place on the electrode surface in an electrochemical cell. The detection system incorporates a biotin-streptavidin capture reaction onto a solid support that permits fast post-PCR product detection at the attomole level. The system precision is within 5% relative standard deviation over a linear dynamic range of greater than three orders of magnitude. In this paper, the principles and features of the electrochemiluminescent-based detection system, together with its application to PCR product quantitation, are described in detail.
已开发出一种用于PCR后产物检测的高灵敏度非同位素系统。基于探针的检测系统利用了在电化学池中电极表面发生的化学发光反应。该检测系统将生物素-链霉亲和素捕获反应整合到固体支持物上，从而能够在阿托摩尔水平快速检测PCR后产物。在大于三个数量级的线性动态范围内，该系统的精密度相对标准偏差在5%以内。本文详细描述了基于电化学发光的检测系统的原理和特点，以及其在PCR产物定量分析中的应用。
