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A sensitive PCR assay system for the quantitation of viral genome equivalents: hepatitis C virus (HCV).

作者信息

Hämmerle T, Falkner F G, Dorner F

机构信息

Immuno AG, Orth/Donau, Austria.

出版信息

Arch Virol. 1996;141(11):2103-14. doi: 10.1007/BF01718218.

DOI:10.1007/BF01718218
PMID:8973526
Abstract

A sensitive and reliable quantitative method has been developed for the detection and quantitation of hepatitis C virus (HCV) target sequences. In this procedure, termed "laser induced fluorescence PCR' (LIF-PCR), reverse transcriptase PCR (RT-PCR) is performed and the PCR products are detected and quantified by laser-induced fluorescence. Precise quantitation of the viral target sequences is accomplished by the use of two calibrators that are amplified by the same set of primers as the target template. A high degree of reliability is achieved by co-processing, co-amplification and co-detection of the calibrators, together with the nucleic acid to be determined. Genome equivalents of HCV containing biological samples, including samples from international test panels, were accurately quantitated with this procedure.

摘要

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本文引用的文献

1
Collaborative study to assess the suitability of an HCV RNA reference sample for detection of an HCV RNA in plasma pools by PCR.
Vox Sang. 1996;70(3):148-51. doi: 10.1111/j.1423-0410.1996.tb01313.x.
2
At least 12 genotypes of hepatitis C virus predicted by sequence analysis of the putative E1 gene of isolates collected worldwide.通过对全球收集的分离株推定的E1基因进行序列分析预测出至少12种丙型肝炎病毒基因型。
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8234-8. doi: 10.1073/pnas.90.17.8234.
3
A high-sensitivity electrochemiluminescence-based detection system for automated PCR product quantitation.一种基于高灵敏度电化学发光的自动化PCR产物定量检测系统。
Biotechniques. 1993 Jul;15(1):152-7.
4
Reliability of polymerase chain reaction for detection of hepatitis C virus.聚合酶链反应检测丙型肝炎病毒的可靠性
Lancet. 1993 Mar 20;341(8847):722-4. doi: 10.1016/0140-6736(93)90488-3.
5
Hepatitis C virus six years on.丙型肝炎病毒六年回顾。
Lancet. 1994 Nov 26;344(8935):1475-9. doi: 10.1016/s0140-6736(94)90293-3.
6
Survey of major genotypes and subtypes of hepatitis C virus using RFLP of sequences amplified from the 5' non-coding region.利用从5'非编码区扩增序列的限制性片段长度多态性对丙型肝炎病毒主要基因型和亚型进行调查。
J Gen Virol. 1995 May;76 ( Pt 5):1197-204. doi: 10.1099/0022-1317-76-5-1197.
7
Clinical application of reverse transcription-polymerase chain reaction for HIV infection.
Clin Lab Med. 1994 Jun;14(2):335-49.
8
The polymerase chain reaction and hepatitis C virus diagnosis.
FEMS Microbiol Rev. 1994 Jul;14(3):229-39. doi: 10.1111/j.1574-6976.1994.tb00093.x.
9
Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis.前瞻性随访的急性和慢性非甲非乙型肝炎输血受者中丙型肝炎病毒抗体的检测
N Engl J Med. 1989 Nov 30;321(22):1494-500. doi: 10.1056/NEJM198911303212202.
10
Quantitation of mRNA by the polymerase chain reaction.通过聚合酶链反应对mRNA进行定量分析。
Proc Natl Acad Sci U S A. 1989 Dec;86(24):9717-21. doi: 10.1073/pnas.86.24.9717.