Muhammad B Y, Kitchen I
School of Biological Sciences, University of Surrey, Guildford, England, U.K.
J Pharmacol Toxicol Methods. 1993 Jun;29(3):139-41. doi: 10.1016/1056-8719(93)90064-l.
Stress-induced antinociception (SIA) which is well characterized in the adult rat can also be observed in young rats, and, by varying swimming times, it can be dissociated into opioid and nonopioid forms. However, swimming ability in the rat does not fully develop until the third postnatal week and this has precluded the study of swim SIA in neonates. We report here the development of a harness device to aid swimming in young rats which we have successfully employed down to the age of 2 days without distress to the animals. Further we have also shown the development of the opioid form of swim SIA in the rat using this device. Swim SIA is absent at days 2 and 5, but at postnatal day 10 a small level of SIA is evident which is reversed by naloxone (10 mg/kg). Swim SIA develops rapidly thereafter, and the adult profile is observed by day 25.
应激诱导的抗伤害感受（SIA）在成年大鼠中已有充分研究，在幼鼠中也可观察到。通过改变游泳时间，SIA可分为阿片类和非阿片类形式。然而，大鼠的游泳能力直到出生后第三周才完全发育，这使得新生儿游泳SIA的研究受到限制。我们在此报告一种用于辅助幼鼠游泳的背带装置的研发，我们已成功将其应用于2日龄的幼鼠，且未对动物造成痛苦。此外，我们还利用该装置展示了大鼠游泳SIA阿片类形式的发育过程。在第2天和第5天未观察到游泳SIA，但在出生后第10天，可明显观察到少量的SIA，纳洛酮（10 mg/kg）可逆转该现象。此后，游泳SIA迅速发展，到第25天观察到其呈现成年大鼠的特征。
