游泳应激诱导幼鼠产生的抗伤害感受。

Swim-stress-induced antinociception in young rats.

作者信息

Jackson H C, Kitchen I

机构信息

Department of Biochemistry, University of Surrey, Guildford.

出版信息

Br J Pharmacol. 1989 Mar;96(3):617-22. doi: 10.1111/j.1476-5381.1989.tb11860.x.

DOI:10.1111/j.1476-5381.1989.tb11860.x

PMID:2720296

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1854388/

Abstract

Opioid and non-opioid mechanisms have been implicated in the phenomenon of stress-induced antinociception in adult rodents. We have studied stress-induced antinociception in developing rats and characterized differences in the neurochemical basis of this effect in pre- and post-weanling animals. 2. Twenty and 25 day old rats were stressed using warm water (20 degrees C) swimming for 3 or 10 min periods and antinociception was assessed by the tail immersion test (50 degrees C). 3. A 3 min swim in 20 and 25 day old rats produced marked antinociception which was blocked by naloxone, Mr 1452, 16-methyl cyprenorphine and levallorphan but not Mr 1453 or N-methyl levallorphan. The delta-opioid receptor antagonist ICI 174,864 attenuated stress-induced antinociception in 25 day old rats but was without effect in 20 day old animals. 4. A 10 min swim in 25 day old rats produced antinociception which was non-opioid in nature. In contrast, antinociception was not observed in 20 day old rats after a 10 min swim-stress. 5. Pretreatment of animals with dexamethasone blocked 3 min swim-stress antinociception in 20 and 25 day old animals but had no effect on antinociception induced by a 10 min swim. 6. Swim-stress-induced antinociception can be observed in young rats and dissociated into opioid and non-opioid types dependent on the duration of swimming stress. The non-opioid type appears to develop more slowly and cannot be observed in preweanling rats. The opioid type is a predominantly mu-receptor phenomenon in preweanling animals but delta-receptor components are observable in postweanling rats.

摘要

阿片类和非阿片类机制与成年啮齿动物应激诱导的抗伤害感受现象有关。我们研究了发育中大鼠的应激诱导抗伤害感受，并对断奶前和断奶后动物这种效应的神经化学基础差异进行了表征。2. 对20日龄和25日龄的大鼠进行温水（20摄氏度）游泳应激3或10分钟，通过尾浸试验（50摄氏度）评估抗伤害感受。3. 20日龄和25日龄大鼠进行3分钟游泳产生明显的抗伤害感受，可被纳洛酮、Mr 1452、16-甲基环丙诺啡和左洛啡烷阻断，但不能被Mr 1453或N-甲基左洛啡烷阻断。δ-阿片受体拮抗剂ICI 174,864减弱了25日龄大鼠应激诱导的抗伤害感受，但对20日龄动物无效。4. 25日龄大鼠进行10分钟游泳产生的抗伤害感受本质上是非阿片类的。相比之下，20日龄大鼠经10分钟游泳应激后未观察到抗伤害感受。5. 用地塞米松预处理动物可阻断20日龄和25日龄动物3分钟游泳应激诱导的抗伤害感受，但对10分钟游泳诱导的抗伤害感受无影响。6. 幼鼠中可观察到游泳应激诱导的抗伤害感受，并根据游泳应激的持续时间分为阿片类和非阿片类。非阿片类似乎发育较慢，在断奶前大鼠中未观察到。阿片类在断奶前动物中主要是μ受体现象，但在断奶后大鼠中可观察到δ受体成分。