Suzuki T, Ohno N, Adachi Y, Yadomae T
Tokyo College of Pharmacy, Japan.
Biol Pharm Bull. 1993 Mar;16(3):223-7. doi: 10.1248/bpb.16.223.
Effects of murine serum (NMS) treatment on (1-->3)-beta-D-glucan inhibitable uptake of zymosan particles (ZYM) (GIZUP) by murine peritoneal macrophages (PM) and the structural specificity of the inhibition were examined. ZYM uptake by PM treated with NMS was enhanced in comparison with those treated with medium, and in a concentration- and incubation time-dependent manner. The enhanced ZYM uptake was significantly reduced by the pretreatment of PM with soluble (1-->3)-beta-D-glucans. These facts suggest that NMS enhances GIZUP. The effect disappeared by the treatment of NMS with gelatin-Sepharose which removed fibronectin (FN) from the serum, suggesting a significant contribution of FN on GIZUP. In addition, the administration of beta-glucan in vivo elevated the concentration of FN in serum by acute phase response and enhanced GIZUP, suggesting the positive contribution of acute phase responses on beta-glucan mediated immunopharmacological activities. Of particular interest, the inhibition was shown by both antitumor active and inactive glucans. These facts suggested that the recognition of beta-glucans by PM, which would proceed at a relatively early period of whole activation pathways, would not be enough to fully activate the host to show antitumor activity.
研究了鼠血清（NMS）处理对鼠腹膜巨噬细胞（PM）对酵母聚糖颗粒（ZYM）的（1→3）-β-D-葡聚糖可抑制摄取（GIZUP）的影响以及抑制作用的结构特异性。与用培养基处理的PM相比，用NMS处理的PM对ZYM的摄取增强，且呈浓度和孵育时间依赖性。用可溶性（1→3）-β-D-葡聚糖预处理PM可显著降低增强的ZYM摄取。这些事实表明NMS增强了GIZUP。用明胶-琼脂糖处理NMS以从血清中去除纤连蛋白（FN）后，该作用消失，表明FN对GIZUP有显著贡献。此外，体内给予β-葡聚糖通过急性期反应提高了血清中FN的浓度并增强了GIZUP，表明急性期反应对β-葡聚糖介导的免疫药理活性有积极贡献。特别有趣的是，抗肿瘤活性和无活性的葡聚糖均表现出抑制作用。这些事实表明，PM对β-葡聚糖的识别在整个激活途径的相对早期进行，但不足以充分激活宿主以显示抗肿瘤活性。
