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流式细胞术在肺炎克雷伯菌荚膜多糖抗吞噬特性研究中的应用。

Application of flow cytometry to the study of antiphagocytic properties of Klebsiella pneumoniae capsular polysaccharide.

作者信息

Ruiz C, Sabio J M, Santos J L, Montes M J, Olivares E G

机构信息

Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Spain.

出版信息

FEMS Immunol Med Microbiol. 1993 Jun;7(1):63-6. doi: 10.1111/j.1574-695X.1993.tb00382.x.

DOI:10.1111/j.1574-695X.1993.tb00382.x
PMID:8364523
Abstract

We used flow cytometry to compare the effects of whole cells and capsular polysaccharides of Klebsiella pneumoniae on the phagocytic ability ot polymorphonuclear leukocytes. Our results showed a light decrease in phagocytic activity in the presence of capsular polysaccharides, but a marked decrease with whole cells. Our findings suggest that the resistance to phagocytosis in these microorganisms is not due exclusively to their capsule, as claimed by other authors.

摘要

我们使用流式细胞术比较了肺炎克雷伯菌的全细胞和荚膜多糖对多形核白细胞吞噬能力的影响。我们的结果显示,在存在荚膜多糖的情况下,吞噬活性略有下降,但与全细胞共同作用时则显著下降。我们的研究结果表明,这些微生物对吞噬作用的抗性并非如其他作者所声称的那样完全归因于它们的荚膜。

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