Litt M, Kramer P, Hauge X Y, Weber J L, Wang Z, Wilkie P J, Holt M S, Mishra S, Donis-Keller H, Warnich L
Oregon Health Sciences University, Portland 97201.
Hum Mol Genet. 1993 Jul;2(7):909-13. doi: 10.1093/hmg/2.7.909.
We have constructed a continuous index map of 25 microsatellite markers on human chromosome 11. The markers have been typed in 40 CEPH families, have heterozygosities of 69% or higher and can be typed by PCR. The odds against inversion of adjacent marker loci order are at least 10(5):1. The sex average map covers a total of 162 cM with no gap exceeding 15 cM. Total lengths for female and male maps are 205 and 123 cM, respectively. By use of a hybrid cell panel or by in situ hybridization, 16 of the markers have also been mapped cytogenetically, providing a good correlation of the index map with the cytogenetic map. The map will facilitate high resolution mapping of additional polymorphic loci and of disease genes on chromosome 11.
我们构建了一张人类11号染色体上25个微卫星标记的连续索引图谱。这些标记已在40个CEPH家系中进行分型，杂合度达到69%或更高，且可通过聚合酶链反应（PCR）进行分型。相邻标记位点顺序发生倒位的概率至少为10⁵:1。性别平均图谱总长162厘摩（cM），最大间隙不超过15 cM。女性和男性图谱的总长度分别为205 cM和123 cM。通过使用杂种细胞板或原位杂交技术，其中16个标记也已进行细胞遗传学定位，从而使索引图谱与细胞遗传学图谱具有良好的相关性。该图谱将有助于对11号染色体上的其他多态性位点和疾病基因进行高分辨率定位。
