O'Connell P, Leach R J, Rains D, Taylor T, Garcia D, Ballard L, Holik P, Weissenbach J, Sherman S, Wilkie P
Department of Pathology, University of Texas Health Science Center at San Antonio 78284.
Genomics. 1994 Dec;24(3):557-67. doi: 10.1006/geno.1994.1666.
Oligonucleotide primers for 125 simple sequence repeat microsatellite-based genetic markers have been assayed by polymerase chain reaction (PCR) in the CEPH reference family panel. These microsatellites include 101 dinucleotide repeats as well as 24 new tetranucleotide repeats. The average heterozygosity of this marker set was 72.4%. Genetic data were analyzed with the genetic mapping package LINKAGE. A subset of these microsatellite markers define a set of 56 uniquely ordered loci (> 1000:1 against local inversion) that span 271 cM. Sixty-seven additional loci were tightly linked to markers on the uniquely ordered map, but could not be ordered with such high precision. These markers were positioned by CMAP into confidence intervals. One hundred thirteen of the microsatellite markers were also tested on a chromosome 3 framework somatic cell hybrid panel that divides this chromosome into 23 cytogenetically defined regions, integrating the genetic and physical maps of this chromosome. The high density, high heterozygosity, and PCR format of this genetically and physically mapped set of markers will accelerate the mapping and positional cloning of new chromosome 3 genes.
利用聚合酶链反应（PCR）对125个基于简单序列重复微卫星的遗传标记的寡核苷酸引物在CEPH参考家系面板中进行了检测。这些微卫星包括101个二核苷酸重复序列以及24个新的四核苷酸重复序列。该标记集的平均杂合度为72.4%。利用遗传图谱软件LINKAGE对遗传数据进行了分析。这些微卫星标记的一个子集定义了一组56个唯一有序的位点（与局部倒位的比例大于1000:1），跨度为271厘摩。另外67个位点与唯一有序图谱上的标记紧密连锁，但无法以如此高的精度进行排序。这些标记通过CMAP定位到置信区间。113个微卫星标记也在一个3号染色体框架体细胞杂种面板上进行了检测，该面板将这条染色体分为23个细胞遗传学定义的区域，整合了这条染色体的遗传图谱和物理图谱。这套经过遗传和物理定位的标记的高密度、高杂合度和PCR形式将加速3号染色体上新基因的定位和位置克隆。
