Boucrot P, Khettab E N, Petit J Y, Welin L
Laboratoire de Pharmacologie et Pharmacocinétique, Nantes.
C R Acad Sci III. 1993;316(3):169-76.
The 1-O-stearoyl-2-O-[3H] arachidonyl-sn-glycero-3-phosphocholine, introduced in the culture medium, was taken up by the peritoneal macrophages activated by the ionophore A 23187. After intracellular phospholipase A2 activity, the [3H] arachidonic acid was found in cells and in extracellular fluids. It also reached the eicosanoid synthesis. When it was introduced in the culture medium with the tritiated phospholipid, the 1, 2 di-O-hexadecyl-rac-glycero-3-phosphocholine, which has a non hydrolysable alkylated structure in the 2 position of the glycerol, inhibited the intracellular phospholipase A2, then contributed to lower the eicosanoid synthesis.
添加到培养基中的1-O-硬脂酰-2-O-[3H]花生四烯酰-sn-甘油-3-磷酸胆碱被离子载体A 23187激活的腹膜巨噬细胞摄取。在细胞内磷脂酶A2发挥作用后，细胞和细胞外液中均发现了[3H]花生四烯酸。它还参与了类二十烷酸的合成。当它与氚标记的磷脂1,2-二-O-十六烷基-rac-甘油-3-磷酸胆碱一起添加到培养基中时，该磷脂在甘油的2位具有不可水解的烷基化结构，可抑制细胞内磷脂酶A2，进而降低类二十烷酸的合成。
