人子宫内膜癌组织中内皮素-1受体结合特性受损。
Impaired binding properties of endothelin-1 receptors in human endometrial carcinoma tissue.
作者信息
Ben-Baruch G, Schiff E, Galron R, Menczer J, Sokolovsky M
机构信息
Department of Obstetrics and Gynecology, Sheba Medical Center, Sackler School of Medicine, Tel-Hashomer, Israel.
出版信息
Cancer. 1993 Sep 15;72(6):1955-8. doi: 10.1002/1097-0142(19930915)72:6<1955::aid-cncr2820720627>3.0.co;2-3.
BACKGROUND
Endothelins, potent stimulators of smooth muscle tissue activity, were recently shown to also function as mitogens for numerous cell types. The authors investigated the properties of endothelin-1 (ET-1) receptors in human endometrial tissue compared with human endometrial carcinoma tissue.
METHODS
Tissue samples from 13 patients with endometrial carcinoma and from 12 women undergoing hysterectomy due to uterus myomatous were obtained immediately after surgical removal. Binding properties of the endothelin receptors were studied using 125I-labeled ET-1.
RESULTS
A significant difference was demonstrated between binding properties of ET-1 receptors of these two groups. The mean maximal density (Bmax) value of the normal endometrial samples was 2029 +/- 341 fmol/mg protein, whereas that of the neoplastic samples was 356 +/- 121 fmol/mg protein. No differences were found, however, between the mean dissociation constant (Kd) values of these groups.
CONCLUSIONS
These results might be compatible with the increased blood flow that characterizes malignant endometrial tissue. However, they do not indicate an important mitogenic role for ET-1 in the development of endometrial cancer.
背景
内皮素是平滑肌组织活性的强效刺激剂，最近研究表明其对多种细胞类型还具有促有丝分裂原的功能。作者比较了人子宫内膜组织和人子宫内膜癌组织中内皮素-1（ET-1）受体的特性。
方法
从13例子宫内膜癌患者以及12例因子宫肌瘤行子宫切除术的女性患者身上获取组织样本，均在手术切除后立即采集。使用125I标记的ET-1研究内皮素受体的结合特性。
结果
两组ET-1受体的结合特性存在显著差异。正常子宫内膜样本的平均最大密度（Bmax）值为2029±341 fmol/mg蛋白质，而肿瘤样本的该值为356±121 fmol/mg蛋白质。然而，两组的平均解离常数（Kd）值没有差异。
结论
这些结果可能与恶性子宫内膜组织中血流量增加相符。然而，它们并未表明ET-1在子宫内膜癌发展中具有重要的促有丝分裂作用。
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