癌胚抗原介导人结肠癌细胞系中γ干扰素诱导的体外细胞聚集：对活跃代谢和完整细胞骨架的需求。

Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-gamma in a human colon cancer cell line: requirement for active metabolism and intact cytoskeleton.

作者信息

Kanai T, Hibi T, Hayashi A, Takashima J, Shiozawa M, Aiso S, Toda K, Iwao Y, Watanabe M, Tsuchiya M

机构信息

Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.

出版信息

Cancer Lett. 1993 Jul 30;71(1-3):109-17. doi: 10.1016/0304-3835(93)90105-i.

DOI:10.1016/0304-3835(93)90105-i

PMID:8364886

Abstract

The homotypic cell aggregation of a carcinoembryonic antigen (CEA) positive colon cancer cell line (Colo 205) was induced in vitro by interferon-gamma (IFN-gamma) treatment. Divalent cations were required for this aggregation, as it was inhibited by EDTA. The partial inhibition by cytochalasin B and the complete inhibition by a mixture of sodium azide and 2-deoxyglucose suggests that the aggregation requires the integrity of cytoskeleton and active metabolism. The expression of CEA was increased in the cytoplasm and on the membrane of Colo 205 by IFN-gamma treatment. Furthermore, this aggregation was inhibited completely by anti-CEA monoclonal antibody (mAb) and partially by mAb against intercellular adhesion molecule-1. This in vitro study suggests that CEA molecule participates in the IFN-gamma induced homotypic adhesion of some CEA positive cancer cells and that IFN-gamma has an important role in the regulation of cell-cell interaction mediated by CEA molecule.

摘要

通过γ-干扰素（IFN-γ）处理在体外诱导癌胚抗原（CEA）阳性结肠癌细胞系（Colo 205）的同型细胞聚集。这种聚集需要二价阳离子，因为它会被乙二胺四乙酸（EDTA）抑制。细胞松弛素B的部分抑制作用以及叠氮化钠和2-脱氧葡萄糖混合物的完全抑制作用表明，聚集需要细胞骨架的完整性和活跃的代谢。通过IFN-γ处理，Colo 205细胞质和细胞膜上的CEA表达增加。此外，这种聚集被抗CEA单克隆抗体（mAb）完全抑制，被抗细胞间粘附分子-1的mAb部分抑制。这项体外研究表明，CEA分子参与了IFN-γ诱导的一些CEA阳性癌细胞的同型粘附，并且IFN-γ在由CEA分子介导的细胞间相互作用调节中起重要作用。