使用流式细胞术对水中隐孢子虫卵囊进行常规监测。

Routine monitoring of Cryptosporidium oocysts in water using flow cytometry.

作者信息

Vesey G, Slade J S, Byrne M, Shepherd K, Dennis P J, Fricker C R

机构信息

Thames Water Utilities Ltd, Spencer House Laboratories, Reading, UK.

出版信息

J Appl Bacteriol. 1993 Jul;75(1):87-90. doi: 10.1111/j.1365-2672.1993.tb03413.x.

DOI:10.1111/j.1365-2672.1993.tb03413.x

PMID:8365959

Abstract

A flow cytometric method for the routine analysis of environmental water samples for the presence of Cryptosporidium oocysts has been developed. It uses a Coulter Epics Elite flow cytometer to examine water samples and to separate oocysts from contaminating debris by cell sorting. The sorted particles are then rapidly screened by microscopy. The method has been evaluated and compared with direct epifluorescence microscopy on 325 river, reservoir and drinking water samples. The technique was found to be more sensitive, faster and easier to perform than conventional epifluorescent microscopy for the routine examination of water samples for Cryptosporidium.

摘要

已开发出一种用于常规分析环境水样中隐孢子虫卵囊的流式细胞术方法。该方法使用库尔特艾匹克思精英流式细胞仪检测水样，并通过细胞分选将卵囊与污染碎片分离。然后通过显微镜对分选的颗粒进行快速筛查。该方法已在325份河流、水库和饮用水样本上进行了评估，并与直接落射荧光显微镜法进行了比较。结果发现，对于水样中隐孢子虫的常规检测，该技术比传统的落射荧光显微镜法更灵敏、更快且更易于操作。