Vesey G, Hutton P, Champion A, Ashbolt N, Williams K L, Warton A, Veal D
School of Biological Sciences, Macquarie University, Sydney, Australia.
Cytometry. 1994 May 1;16(1):1-6. doi: 10.1002/cyto.990160102.
Cryptosporidium and Giardia are common causes of waterborne disease. The currently used methods of detecting these organisms in water rely on filtration capture, immunofluorescence labelling, and epifluorescence microscopy. These methods are inefficient, labour intensive, and require a highly skilled microscopist. We describe an alternative technique using flocculation concentration, followed by flow cytometry with fluorescence activated cell sorting. Environmental samples were analysed, and protozoan-like particles were sorted and collected before confirmation with epifluorescence microscopy. The technique was found to be significantly more sensitive and considerably faster than the conventional methods.
隐孢子虫和贾第虫是水源性疾病的常见病因。目前用于检测水中这些生物体的方法依赖于过滤捕获、免疫荧光标记和落射荧光显微镜检查。这些方法效率低下、劳动强度大，并且需要技术高超的显微镜技术人员。我们描述了一种替代技术，该技术采用絮凝浓缩，随后进行带有荧光激活细胞分选的流式细胞术。对环境样本进行了分析，在通过落射荧光显微镜确认之前，对类原生动物颗粒进行了分选和收集。结果发现，该技术比传统方法明显更灵敏且速度快得多。
