Sabourin C L, Kusewitt D F, Fry R J, Ley R D
Center for Photomedicine, Lovelace Medical Foundation, Albuquerque, NM 87108.
J Comp Pathol. 1993 May;108(4):343-59. doi: 10.1016/s0021-9975(08)80206-x.
Chronic exposure of the South American opossum, Monodelphis domestica, to ultraviolet radiation (UVR) induced 154 primary tumours of the cornea in 152 eyes. Tumours developed gradually; frank neoplasia was preceded by non-neoplastic proliferation of corneal stromal fibroblasts (keratocytes) and extensive neovascularization. Histologically, the majority of tumours (134 of 154) appeared to be fibrosarcomas arising from keratocytes, but about 12 per cent of the tumours (18 of 154) had a highly vascular appearance, suggesting haemangiosarcoma. In two eyes, squamous cell carcinomas overlay mesenchymal tumours. Ultrastructural features of UVR-induced corneal tumours were consistent with tumours, and cultured skin fibroblasts expressed high content of messenger RNA for the intermediate filament vimentin; no cytokeratin messenger RNA was detected in these cells and cell lines. Based upon their light microscopic, ultrastructural, and intermediate filament biosynthetic characteristics, the majority of UVR-induced corneal tumours in M. domestica appeared to be fibrosarcomas. Haemangiosarcomas constituted a smaller proportion of the tumours, and squamous cell carcinomas were very rare.
将南美负鼠（Monodelphis domestica）长期暴露于紫外线辐射（UVR）下，导致152只眼睛出现了154个原发性角膜肿瘤。肿瘤逐渐发展；在出现明显的肿瘤形成之前，角膜基质成纤维细胞（角膜细胞）会发生非肿瘤性增殖并伴有广泛的新生血管形成。组织学上，大多数肿瘤（154个中的134个）似乎是起源于角膜细胞的纤维肉瘤，但约12%的肿瘤（154个中的18个）具有高度血管化外观，提示为血管肉瘤。在两只眼睛中，鳞状细胞癌覆盖在间充质肿瘤之上。UVR诱导的角膜肿瘤的超微结构特征与肿瘤一致，培养的皮肤成纤维细胞表达中间丝波形蛋白的信使RNA含量很高；在这些细胞和细胞系中未检测到细胞角蛋白信使RNA。基于其光学显微镜、超微结构和中间丝生物合成特征，南美负鼠中大多数UVR诱导的角膜肿瘤似乎是纤维肉瘤。血管肉瘤在肿瘤中所占比例较小，鳞状细胞癌非常罕见。
