Pschorn U, Körperich H, Heymans L, Subramanian S, Kuhn W
Boehringer Ingelheim KG, Department of Pharmacology, Germany.
Magn Reson Med. 1993 Aug;30(2):174-82. doi: 10.1002/mrm.1910300205.
Magnetic resonance imaging has been used to follow the time course of lesions induced in the rat brain as an animal model for characterization of the volume of the lesion. The dispersion in spin-spin relaxation has been used to characterize the nature of the brain lesion. Parameter selective estimation of T2, quantitative determination of the lesion size and volume selective in vivo proton spectroscopy have been employed for the purpose. The work has been carried out on rats which were subject to lesioning by ibotenic acid as a model for excitotoxicity and also on rats which received doses of ibotenic acid and subsequent doses of the NMDA antagonist drug MK 801 (dizocilpine). The time course of the progress of the lesions in untreated animals and the effect of neuroprotection by MK 801 was continuously monitored in all test animals. Further, a relatively new inhalation anesthetic agent, isoflurane, has been employed. A more logical and semiquantitative T2 bandwidth demarkation useful in distinguishing different degrees of lesioning from the onset and up to the 'edema' stage through penumbra (mild lesion), medium degree lesion and severe lesion has been proposed.
磁共振成像已被用于追踪大鼠脑中诱导损伤的时间进程，以此作为一种动物模型来表征损伤体积。自旋 - 自旋弛豫的离散已被用于表征脑损伤的性质。为此采用了T2的参数选择性估计、损伤大小的定量测定以及体内体积选择性质子光谱分析。这项工作是在通过注射鹅膏蕈氨酸造成损伤作为兴奋性毒性模型的大鼠身上进行的，同时也在接受了鹅膏蕈氨酸剂量以及随后NMDA拮抗剂药物MK 801（地佐环平）剂量的大鼠身上开展。在所有受试动物中持续监测未治疗动物损伤进展的时间进程以及MK 801的神经保护作用。此外，还使用了一种相对较新的吸入麻醉剂异氟烷。已经提出了一种更合理的半定量T2带宽划分方法，该方法有助于从损伤开始直至“水肿”阶段，通过半暗带（轻度损伤）、中度损伤和重度损伤来区分不同程度的损伤。
