Ben-Horin N, Hazvi S, Bendel P, Schul R
Department of Neurobiology, Weizmann Institute, Rehovot, Israel.
Brain Res. 1996 Apr 29;718(1-2):97-104. doi: 10.1016/0006-8993(96)00058-3.
We have used magnetic resonance imaging (MRI) of the living rat brain to longitudinally analyze the ontogenesis of an ibotenic acid lesion targeted at the piriform cortex. The MRI data were systematically compared with data obtained from a battery of histopathological techniques, including Nissl stain, hematoxylin stain, and a stain for cytochrome oxidase activity. Two days after the lesioning, widespread and heterogeneous damage was detected in, around and distant from the toxin-targeted area. Some damage apparently diminished within approximately 10 days, whereas other damage remained throughout the length of this study (60 days). We found that the small-animal MRI technology used by us is useful in determining the initial, transient impact of surgery and neurotoxic lesioning, and in delineating the gross effects of the lesion over time. This is particularly useful for early elimination of animals from the protocol of physiological and behavioral experiments in which the lesion exceeds the target area. Our data also indicate that, in order to avoid confounding effects of transient post-lesioning phenomena, behavioral and physiological tests should be carried out in neurotoxically lesioned animals > 2 weeks after infliction of the lesion.
我们利用活体大鼠脑的磁共振成像（MRI）对靶向梨状皮质的鹅膏蕈氨酸损伤的个体发生进行了纵向分析。将MRI数据与通过一系列组织病理学技术获得的数据进行了系统比较，这些技术包括尼氏染色、苏木精染色以及细胞色素氧化酶活性染色。损伤后两天，在毒素靶向区域及其周围和远处检测到广泛且异质性的损伤。一些损伤在大约10天内明显减轻，而其他损伤在本研究的整个时间段（60天）内一直存在。我们发现，我们所使用的小动物MRI技术可用于确定手术和神经毒性损伤的初始、短暂影响，并描绘损伤随时间的总体效应。这对于在生理和行为实验方案中早期剔除损伤超出目标区域的动物尤为有用。我们的数据还表明，为避免损伤后短暂现象的混杂效应，应在神经毒性损伤动物损伤后>2周进行行为和生理测试。
