Andersson-Engels S, Ankerst J, Johansson J, Svanberg K, Svanberg S
Department of Physics, Lund Institute of Technology, Sweden.
Photochem Photobiol. 1993 Jun;57(6):978-83. doi: 10.1111/j.1751-1097.1993.tb02958.x.
Laser-induced fluorescence was used to characterize the localization of intravenously administered benzoporphyrin derivative-monoacid (BPD-MA) 3 h postinjection in different rat tissue types, including an induced experimental malignant tumor. A comparison of the fluorescence properties and demarcation potential between the newer sensitizer BPD-MA and four other substances, hematoporphyrin (HP), polyhematoporphyrin ester (PHE), tetrasulfonated phthalocyanine (TSPc) and the commercially available Photofrin earlier investigated, is included. The fluorescence light was induced with a nitrogen laser, emitting at 337 nm. The fluorescence spectrum in the region 380-750 nm was analyzed by a polychromator equipped with a diode array detector. The demarcation potential between tumor and surrounding tissue in terms of fluorescence signal for the tumor model used was 2:1 for BPD-MA. In comparison with the other drugs, HP shows about the same demarcation potential, whereas Photofrin and PHE exhibit about 3 times better and TSPc about 1.5 times better demarcation. By also employing the endogenous tissue fluorescence signature the contrast was enhanced by a factor of about 2 for each of the five drugs.
采用激光诱导荧光技术，对静脉注射苯卟啉衍生物单酸（BPD-MA）3小时后在不同大鼠组织类型（包括诱导实验性恶性肿瘤）中的定位进行了表征。文中还比较了新型敏化剂BPD-MA与其他四种物质（血卟啉（HP）、聚血卟啉酯（PHE）、四磺酸酞菁（TSPc）以及之前研究过的市售光卟啉）之间的荧光特性和分界潜力。用发射波长为337nm的氮激光诱导荧光。用配备二极管阵列探测器的多色仪分析380 - 750nm区域的荧光光谱。对于所使用的肿瘤模型，BPD-MA的肿瘤与周围组织之间基于荧光信号的分界潜力为2:1。与其他药物相比，HP的分界潜力大致相同，而光卟啉和PHE的分界效果约好3倍，TSPc的分界效果约好1.5倍。通过利用组织内源性荧光特征，这五种药物的对比度均提高了约2倍。
