Andersen J M, Nervi F O, Dietschy J M
Biochim Biophys Acta. 1977 Feb 23;486(2):298-307. doi: 10.1016/0005-2760(77)90025-x.
The increase in the mass of cholesterol esters in the liver was used to estimate hepatic net uptake rates of cholesterol from various serum and intestinal lipoprotein fractions. Initial uptake rates equalled essentially zero for high density serum lipoproteins and for large chylomicrons while administration of both low density serum lipoproteins and smaller chylomicrons produced a significant increase in hepatic cholesterol ester levels. The rate of uptake of both serum lipoprotein fractions did not change over a 5 h interval after injection: in contrast, the rates of uptake of the intestinal fractions increased 10-25-fold during this interval. Circulation of large chylomicrons in functionally eviscerated rats markedly increased the rate of hepatic cholesterol uptake when these metabolized lipoproteins were reinjected into recipient animals. Uptake of cholesterol from intestinal lipoproteins was essentially a linear function of the amount of chylomicrons administered to the animals and was independent of the level of circulating serum cholesterol and the rate of hepatic cholesterogenesis. These observations are consistent with the view that the liver is capable of taking up cholesterol from chylomicron remnants and, at significantly lower rate, low density serum lipoproteins.
肝脏中胆固醇酯质量的增加被用于估计肝脏从各种血清和肠道脂蛋白组分中摄取胆固醇的净速率。高密度血清脂蛋白和大乳糜微粒的初始摄取速率基本为零，而给予低密度血清脂蛋白和较小的乳糜微粒均会使肝脏胆固醇酯水平显著升高。注射后5小时内，两种血清脂蛋白组分的摄取速率均未改变；相比之下，在此期间肠道组分的摄取速率增加了10至25倍。当将这些代谢后的脂蛋白重新注入受体动物体内时，在功能性去脏大鼠体内循环的大乳糜微粒显著提高了肝脏胆固醇摄取速率。从肠道脂蛋白中摄取胆固醇基本上是给予动物的乳糜微粒量的线性函数，并且与循环血清胆固醇水平和肝脏胆固醇生成速率无关。这些观察结果与以下观点一致，即肝脏能够从乳糜微粒残粒中摄取胆固醇，并且以显著较低的速率从低密度血清脂蛋白中摄取胆固醇。
