Awogun I A
Stain Technol. 1978 Jul;53(4):187-90. doi: 10.3109/10520297809111463.
A rapid whole mount staining method is described to identify and differentiate microfilariae without elaborate processing. A single solution combining Hoyer's mounting medium and hematoxylin stain facilitates light microscopic examination of nuclei and sheaths of microfilariae. The new technique stains microfilariae adequately in three to seven minutes at 60--64 C making the method preferable to conventional methods that may take as long as 45 to 60 minutes. Lantern heat may be used to heat slides in rural areas with good results.
本文描述了一种快速整体染色方法，无需复杂处理即可识别和区分微丝蚴。一种将霍耶尔封固剂和苏木精染色剂结合的单一溶液便于对微丝蚴的细胞核和鞘进行光学显微镜检查。这项新技术在60 - 64摄氏度下三到七分钟就能充分染色微丝蚴，使该方法优于可能需要长达45到60分钟的传统方法。在农村地区可用灯笼加热载玻片，效果良好。
