用非同位素聚合酶链反应-连接酶链反应分析法检测单核细胞增生李斯特菌。
Detection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay.
作者信息
Wiedmann M, Barany F, Batt C A
机构信息
Department of Food Science, Cornell University, Ithaca, New York 14853.
出版信息
Appl Environ Microbiol. 1993 Aug;59(8):2743-5. doi: 10.1128/aem.59.8.2743-2745.1993.
A polymerase chain reaction (PCR)-coupled ligase chain reaction (LCR) assay for the specific detection of Listeria monocytogenes (M. Wiedmann, J. Czajka, F. Barany, and C. A. Batt, Appl. Environ. Microbiol. 58:3443-3447, 1992) has been modified for detection of the LCR products with a nonisotopic readout. When a chemiluminescent or a colorimetric substrate for the nonisotopic detection of the LCR products was used, the PCR-coupled LCR gave a sensitivity of 10 CFU of L. monocytogenes. The detection method with the chemiluminescent substrate Lumi-Phos 530 permitted detection of the LCR products in less than 3 h, so that the whole assay can be completed within 10 h.
一种用于特异性检测单核细胞增生李斯特菌的聚合酶链反应（PCR）偶联连接酶链反应（LCR）分析方法（M. 维德曼、J. 恰伊卡、F. 巴拉尼和C. A. 巴特，《应用与环境微生物学》58:3443 - 3447，1992年）已被改进，用于通过非同位素读数检测LCR产物。当使用用于LCR产物非同位素检测的化学发光或比色底物时，PCR偶联LCR对单核细胞增生李斯特菌的检测灵敏度为10 CFU。使用化学发光底物Lumi-Phos 530的检测方法能够在不到3小时内检测到LCR产物，因此整个分析可在10小时内完成。
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