Thornthwaite J T, Leif R C
Stain Technol. 1978 Jul;53(4):199-204. doi: 10.3109/10520297809111466.
The alcian blue dye exclusion method for glutaraldehyde-fixed cells has been utilized with "centrifugal cytology" to prepare permanent records of the viability of individual cells present in suspensions. The viability of spleen cell suspensions separated by linear bovine serum albumin density gradient centrifugation has been measured with this method. Combined light and scanning electron microscopy of nonviable and viable cells demonstrated membrane alterations in alcian blue-stained nonviable cells, while viable cells were spherical and displayed uniform surface features.
用于戊二醛固定细胞的阿尔辛蓝染料排除法已与“离心细胞学”结合使用，以制备悬浮液中单个细胞活力的永久记录。已用此方法测量了通过线性牛血清白蛋白密度梯度离心分离的脾细胞悬液的活力。对非存活细胞和存活细胞进行的光镜和扫描电镜联合观察显示，阿尔辛蓝染色的非存活细胞存在膜改变，而存活细胞呈球形且表面特征均匀。
