基于针对半乳糖特异性粘附素的单克隆抗体，采用粪便酶联免疫吸附测定法诊断致病性溶组织内阿米巴感染。

Diagnosis of pathogenic Entamoeba histolytica infection using a stool ELISA based on monoclonal antibodies to the galactose-specific adhesin.

作者信息

Haque R, Kress K, Wood S, Jackson T F, Lyerly D, Wilkins T, Petri W A

机构信息

International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.

出版信息

J Infect Dis. 1993 Jan;167(1):247-9. doi: 10.1093/infdis/167.1.247.

DOI:10.1093/infdis/167.1.247

PMID:8369019

Abstract

Monoclonal antibodies (MAbs) directed against pathogen-specific epitopes of the galactose adhesin of Entamoeba histolytica were used in an ELISA to detect antigen from pathogenic E. histolytica. Single stool specimens from 74 patients in Bangladesh were used. The ELISA for pathogenic E. histolytica was positive in all 12 stool specimens with pathogenic amebae subsequently cultured, in no stool specimens with nonpathogenic E. histolytica and in 2 of 40 stools with other or no intestinal parasites detected. Specificity and sensitivity of the assay for pathogenic E. histolytica were 97% and 100%, respectively. These preliminary data offer promise for an ELISA using MAbs to the galactose adhesin as a rapid and sensitive means to detect the presence of pathogenic E. histolytica infection in stool specimens.

摘要

针对溶组织内阿米巴半乳糖黏附素病原体特异性表位的单克隆抗体（MAbs）被用于酶联免疫吸附测定（ELISA）中，以检测来自致病性溶组织内阿米巴的抗原。使用了孟加拉国74名患者的单一粪便样本。致病性溶组织内阿米巴的ELISA在随后培养出致病性阿米巴的所有12份粪便样本中呈阳性，在非致病性溶组织内阿米巴的粪便样本中均为阴性，在检测出其他或无肠道寄生虫的40份粪便样本中有2份呈阳性。该致病性溶组织内阿米巴检测方法的特异性和敏感性分别为97%和100%。这些初步数据表明，使用针对半乳糖黏附素的单克隆抗体进行ELISA有望成为一种快速、灵敏的手段，用于检测粪便样本中致病性溶组织内阿米巴感染的存在。