通过亲和层析法纯化大鼠肠道内维生素B12-内因子复合物受体
Purification of rat intestinal receptor for intrinsic factor-vitamin B-12 complex by affinity chromatography.
作者信息
Yamada S, Itaya H, Nakazawa O, Fukuda M
出版信息
Biochim Biophys Acta. 1977 Feb 28;496(2):571-5. doi: 10.1016/0304-4165(77)90340-3.
PMID:836908
Abstract
Rat intrinsic factor was bound to vitamin B-12-Sepharose to produce intrinsic factor-vitamin B-12-Sepharose. Intestinal receptor for intrinsic factor-vitamin B-12 complex was purified from rat ileal extract by affinity chromatography using the intrinsic factor-vitamin B-12-Sepharose as an affinity adsorbent with recovery of 48.5% and specific activity increased 335 fold of original sample.
摘要
将大鼠内因子与维生素B-12-琼脂糖结合，制成内因子-维生素B-12-琼脂糖。以内因子-维生素B-12-琼脂糖作为亲和吸附剂，通过亲和色谱法从大鼠回肠提取物中纯化内因子-维生素B-12复合物的肠道受体，回收率为48.5%，比活性比原始样品提高了335倍。
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